The understanding of molecular mechanisms and gene regulation involved in pigmentation and inflammatory processes, is needed to identify new therapeutic targets. For gene expression quantification, RNA is extracted from skin biopsies exposed and unexposed to UV radiation.
Compared to classical lysis (chaotropics ions + ß- Mercapto ethanol + Vortex), RNA yield increased from 2 to 4 fold (Nanodrop quatification). RNA quality was assessed using a 2100 Bioanalyser. RNA Integrity Number (RIN) is over 8, meaning good quality.