RNA extraction from Human Skin Biopsies - Bertin Instruments

    Biomedical & Health

    RNA extraction from Human Skin Biopsies

    Sources: Mouchet1* 2N., Galibert1 M. D. I) UMR 6061 CNRS, Rennes (France) 2) Proclaim, Saint-Gregoire (France)

    Context

    The understanding of molecular mechanisms and gene regulation involved in pigmentation and inflammatory processes, is needed to identify new therapeutic targets. For gene expression quantification, RNA is extracted from skin biopsies exposed and unexposed to UV radiation.

    Results

    Compared to classical lysis (chaotropics ions + ß- Mercapto ethanol + Vortex), RNA yield increased from 2 to 4 fold (Nanodrop quatification). RNA quality was assessed using a 2100 Bioanalyser. RNA Integrity Number (RIN) is over 8, meaning good quality.

     

    Cryolys Option should be used to keep the samples at low temperature.

    Cryolys Option should be used to keep the samples at low temperature.

     

    Chromatograms from 2 patients A and B.

    Chromatograms from 2 patients A and B.

    ANY QUESTIONS? CONTACT AN EXPERT

    Please fill in this form if you wish to contact our sales team or receive further information.

    My Account
    Forgot password ?

    Add a question !

    Add a question