RNA extraction from ixoidid ticks with Precellys24 - Bertin Instruments

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    RNA extraction from ixoidid ticks with Precellys24

    Sources: Korean J parasitol vol.52,No. 2:217-220, April 2014

    Context

    Tick-borne disease surveillance is becoming increasingly important as zoonotic tick-borne pathogens are recognized to affect man and wild and domestic animals worldwide. Dragging vegetation for questing ticks are often employed where ticks are present and provides information on habitats and seasonal life stage distributions.
    The purpose of the present study was to provide estimates of the distribution of life cycle stages during periods when ixoidid ticks are active and their infection status with SFTS virus.
    The Precellys24 has been used to extract RNA from pools of ticks larvae, nymphs and adults in order to examine the infection status with severe fever with thrombocytopenia syndrome (SFTS) virus using RT-PCR.

    Results

    Table 1 : Morphologic identification of ticks collected from natural/city parks from June to October 2013 and assayed for tick-borne severe fever with SFTS virus, by species and developmental stage


    All of the 63 pools of 796 ticks (larvae, nymphs, and adults) belonged to 3 species, and none were positive for SFTS virus by conventional RT-PCR.

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