RNA Extraction from Mouse Tissues using the Minilys - Bertin Instruments

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    RNA Extraction from Mouse Tissues using the Minilys

    Sources: Cystic Fibrosis and Pulmonary Research Center, University of North Carolina at Chapel Hill, North Carolina, USA

    Context

    Our research focuses on studying the normal physiology of airway surface liquids and failure of this system, which results in cystic fibrosis and chronic pulmonary disease (COPD). We examine RNA expression of MUC5AC in various mouse tissues, which is associated with COPD development. The aim of this study is to improve workflow efficiency by comparing the performance of two instruments for mouse lung and tracheal tissue homogenization.

    Results

    Replicate samples from lung and tracheal mouse tissue were prepared and homogenized on the Minilys and Competitor B. Visual images were captured for lung tissue before and after homogenization to compare efficiency. Figure 1 demonstrates that Competitor B yields incomplete homogenization after running a protocol at the unit’s maximum speed. Figure 2 shows that the lung tissues were thoroughly homogenized on the Minilys.

    Lung tissue before (A) and after homogenization (B) on Competitor B. The blue arrows indicate unhomogenized tissue.

    Figure 1 :Lung tissue before (A) and after homogenization (B) on Competitor B. The blue arrows indicate unhomogenized tissue.

     

    mouse minilys
    Figure 2. Lung tissue before (A) and after homogenization (B) on the Minilys.

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