RNA extraction from Streptomyces (Actinobacteria) - Bertin Instruments

Microbiology

RNA extraction from Streptomyces (Actinobacteria)

Sources: Lab. Microbial Metabolism – Shanghai Jiao Tong University

Context

Validamycin is a non-systemic antibiotic with fungicide action. It is most effective against damping-off diseases and is used for protecting seedling and clone cuttings. Our lab is focused on Functional Analysis of the Validamycin Biosynthetic Gene Cluster and Engineered Production of Validoxylamine-A. This study compares the efficiency of total RNA isolation in using the Precellys®24 vs. Enzymatic lysis.

Results

Total RNA extracted is visualized by ethidium bromide staining and UV Illumination. RNA quantity was found to be 2.5 times higher for Precellys®24 samples when compared to Enzymatic lysis.
rna extraction from streptomyces
Total RNA extracted from Streptomyces

The quantity of extracted RNA when using the Precellys®24 is almost twice the amount with enzymatic lysis

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