RNA extraction from torafugu pufferfish with Minilys - Bertin Instruments

    Environment

    RNA extraction from torafugu pufferfish with Minilys

    Sources: The Univ. of Tokyo, Graduate School of Agricultural and Life Sciences, Laboratory of Marine Biochemistry, Japan

    Context

    Our laboratory focuses on the mechanisms involved in the biological processes in relation to energy metabolism, stress responses and cell signaling pathway for sustainable and effective utilization of aquatic resources. The extraction of RNA from livers or muscles of various fishes is a part of laboratory’s daily work.

    Results

    The quality of extracted RNA from torafugu pufferfish was good (Fig.1). For the torafugu pufferfish muscle which is exceptionally hard among the fish muscle, 5000 rpm 2x 30sec gave very good RNA yield (Table1). The 4000 rpm treatment was not enough (the homogenate looked somewhat granular). The RNA concentration extracted from the fish liver using Minilys was somewhat smaller than the amount of RNA extracted using Precellys®24.

     

    Figure 1: Agarose gel electrophoresis of the RNA extracted from torafugu pufferfish.

    Figure 1: Agarose gel electrophoresis of the RNA extracted from torafugu pufferfish.

     

    Table 1: concentration of RNA extracted (µg/µl).

    Table 1: concentration of RNA extracted (µg/µl).

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