RNA Extractions of Microorganisms - Bertin Instruments

    Microbiology

    RNA Extractions of Microorganisms

    Sources: Laboratory of molecular Genetic of theNeurotransmission, CNRS of Paris

    Context

    Within the context of research on genetic analysis, molecular biology brings rapid and reliable answers concerning an eventual pathogen germs contamination (identification and detection). The sample preparation is an important step, which needs to be efficient without degrading the material, particularly DNA, RNA, or proteins.

    Results

    Results were conducted in collaboration with LGN (Laboratory of Molecular Genetic of the Neurotransmission). E.coli and spores of B.globigii were lysed with Precellys kit compared to standard procedures. RNA extraction was performed from the 2 microorganisms following each sample preparation.

     

    1. E.coli (Precellys) 2. E.coli (standard) 3. B.globigii spores (Precellys) 4. B.globigii spores (Standard) LGN Paris, April 2004 RNA extraction after lysis

    1. E.coli (Precellys) 2. E.coli (standard) 3. B.globigii spores (Precellys) 4. B.globigii spores (Standard) LGN Paris, April 2004 RNA extraction after lysis

     

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