Sample extraction for quantification of mouldantigen - Bertin Instruments

    Microbiology

    Sample extraction for quantification of mouldantigen

    Sources: Institut für Prävention und Arbeitsmedizin, Institut der Ruhr-Universität Bochum (IPA), Bürkle-de-la-Camp Platz, Bochum, Germany

    Context

    To assess exposure to mould antigens, enzyme immunoassays for Aspergillus fumigatus and Penicillium chrysogenum antigens had been developed. After different extraction procedures with Precellys homogenization or mixing only, the antigen yields of airborne dust samples were compared and one method was defined [1].

     

     

    Results

    For extraction of airborne dust parallel sampled on 10 Teflon filters in composting plants, the SK38 – 6000 rpm homogenization procedures (mixed only, Precellys homogenization, with filter removed or not) were applied. With Precellys SK38 homogenization, A. fumigatus and P. chrysogenum antigen yields were higher than by mixing only irrespective of the mode of filter removal. Higher antigen amounts were obtained for both volumes of homogenization: 2mL (Fig. 1) or 7mL kit, with 1mL or 3mL of buffer, respectively.

     

    Figure 1: In 5 composting plants, airborne dust was sampled on Teflon filters with a Parallel sampler. The Aspergillus fumigatus (a) or Penicillium chrysogenum (b) antigen yields after extraction in 2 ml Precellys tubes were measured by enzyme immunoassays (mean of 2 filters per procedure).

    Figure 1: In 5 composting plants, airborne dust was sampled on Teflon filters with a Parallel sampler. The Aspergillus fumigatus (a) or Penicillium chrysogenum (b) antigen yields after extraction in 2 ml Precellys tubes were measured by enzyme immunoassays (mean of 2 filters per procedure).

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