Spleen (Frozen) – Mouse – Protein Extraction - Bertin Instruments

    Protocol

    Spleen (Frozen) – Mouse – Protein Extraction

    Sample type

    Animal

    Buffer

    1 ml of lysis buffer (10 mM Tris-HCl pH 7.05, 50 mM NaCl, 30 mM NaPPi, 50 mM NaF, 2 mM EDTA, 1 % Triton X-100, 0.1 mM sodium vanadate, 1 mM phenylmethylsulfonyl fluoride, protease inhibitor cocktail (Roche))

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