Homogenization of biological samples for protein extraction using Precellys® Evolution and Cryolys® Evolution ensures a high yield of protein recovery and prevents protein from heat degradation during this process. However, all proteins, including endogenous proteases, being protected during this step, a subsequent degradation of the proteins of interest can occur during and after the homogenization. Therefore, it is highly advised to protect proteins of interest from degradation by endogenous proteases released by the sample.
In this Application Note, we highlight how the use of the Protein Safe Precellys® Lysing kits ensures an efficient protection of samples from endogenous proteases in different kinds of tissue homogenates.
Fluorescence levels reflecting the proteases activity levels were measured in the different samples following the method described previously. The different activity levels measured for a same sample extracted with Classical Precellys® Lysing kit or the Protein Safe Lysing kit are represented in the figures 1 to 4 for Heart, Liver, Lung and Brain.