Our platform is specialized in extraction of nucleic acids from tumoral tissues intended to be analyzed by microarray and sequencing. In this study, nucleic acids have been extracted from important series of cartilage tumors. Although DNA extraction from cartilage samples is common, the extraction of high quality RNA is still a challenge. In this way, the Precellys Dual was evaluated to increase quality of extracted RNA from cartilage tumors.
Qualification of RNA and miRNA is based on some criteria as 28s/18s ratio, RIN and Agilent 2100 Bioanalyzer profiles. Figures 1 and 2 show the results of RNA and miRNA qualifications extracted from a cartilage tumor. Characteristics of total RNA are reported in Table 1. RNA and miRNA quality and integrity are optimal to microarray and sequencing analysis.