Total RNA extraction from Cartilage tumors - Bertin Instruments

    Cancer research

    Total RNA extraction from Cartilage tumors

    Sources: Quality Control Platform of Nucleic Acids, Programme Carte d’Identité des Tumeurs (CIT), Ligue Nationale Contre le Cancer, Paris, France.

    Context

    Our platform is specialized in extraction of nucleic acids from tumoral tissues intended to be analyzed by microarray and sequencing. In this study, nucleic acids have been extracted from important series of cartilage tumors. Although DNA extraction from cartilage samples is common, the extraction of high quality RNA is still a challenge. In this way, the Precellys Dual was evaluated to increase quality of extracted RNA from cartilage tumors.

    Results

    Qualification of RNA and miRNA is based on some criteria as 28s/18s ratio, RIN and Agilent 2100 Bioanalyzer profiles. Figures 1 and 2 show the results of RNA and miRNA qualifications extracted from a cartilage tumor. Characteristics of total RNA are reported in Table 1. RNA and miRNA quality and integrity are optimal to microarray and sequencing analysis.

    Profile of RNA extracted from a cartilage tumor on Agilent 2100 Bioanalyzer. Table1: Characteristics of total RNA extracted from a cartilage tumor.

    Profile of RNA extracted from a cartilage tumor on Agilent 2100 Bioanalyzer. Table1: Characteristics of total RNA extracted from a cartilage tumor.

     

    Profile of miRNA extracted from a cartilage tumor on Agilent 2100 Bioanalyzer.

    Profile of miRNA extracted from a cartilage tumor on Agilent 2100 Bioanalyzer.

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