Tumor – Mouse – Protein Extraction - Bertin Instruments

    Protocol

    Tumor – Mouse – Protein Extraction

    Sample type

    Animal

    Buffer

    lysis buffer [10 mM Tris pH 7.5 (InVitrogen 15567−027), 100 mM NaCl (Sigma S5150), 1 mM EDTA pH 8 (InVitrogen 15575−038), 1 mM EGTA pH 8 (Sigma E4378), 1% Triton (Sigma T9284), 1 mM NaF (Sigma S7920), 20 mM Na4P2O7 (Sigma S6422), 1 mM activated Na3VO4 (Sigma S6508), 10% glycerol (Fisher Scientific G/0650/17) supplemented with protease inhibitor mix (Roche 11836145001)]

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