Yeast Candida Albicans – Fungi – Protein Extraction

Fungi

Lysis buffer (50mM Tris-HCl, pH 5; 10% glycerol; 1% Triton-X100; 0.1% SDS; 0.15M NaCl; 50mM NaF; 10mM Sodium orthovanadate; 50mM ?-glycerol phosphate; 50mM sodium pyrophosphate; 5mM EDTA, pH 8; 1mM PMSF; 1x protease inhibitor cocktail, Sigma)