Yeast Saccharomyces Cerevisiae – Fungi – RNA / DNA / Protein Extraction - Bertin Instruments

    Protocol

    Yeast Saccharomyces Cerevisiae – Fungi – RNA / DNA / Protein Extraction

    Sample type

    Fungi

    Buffer

    0.4 ml of lysis buffer prepared in fresh DEPC treated water
    Lysis buffer (10 mM Tris-HCl pH7.4, 100 mM NaCl, 30 mM MgCl2, 200 ug/ml heparin, 50 μg/ml cycloheximide, 1 mM DTT, 1 μl/ml RNase inhibitor, EDTA-free protease inhibit cocktail 1 tablet in 10 ml buffer)

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