Choosing your lysing kit - Bertin Instruments

Choosing your lysing kit

The disruption of samples is the first step in the process of isolation and/or quantification of nucleic acids, proteins, and analytes. For this reason, choosing the right homogenization protocol will have a great impact on the quality and reliability of results for most biological analytical workflows.

Bertin Technologies offers a range of robust, efficient, and flexible homogenizers and lysing kits. Our products are based on bead beating technology that can grind any tissues and lyse any cells prior to DNA, RNA, and protein analysis.

Homogenization protocols can vary significantly depending on the nature and the quantity of the sample. Our team of scientists have designed a comprehensive range of lysing kits that provide researchers with an optimized solution for each of their workflows.

When using the 3D bead-beating technology, the choice of the bead size and material can greatly affect the disruption energy applied to the sample. The optimal bead density and bead material vary depending on the sample hardness. For harder samples, the bead material should be more resistant. For example, seeds can be homogenized with metal beads. For a given sample hardness, the bead size should be proportional to the target size. For example, bacteria vs 0.1mm bead. Sometimes, mixing the bead sizes and/or material increases the efficiency of the homogenization.

See Table 1 to identify a suitable kit for your sample

The Precellys lysing kits offer the best versatility of volumes and grinding material to address any type of samples

Optimize your homogenization process

Please contact us to request free samples.

Our field application specialists can help you identify the best lysing kit and protocol for your sample.

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