With more than several thousand publications in less than 2 years, CRISPR-Cas9 revolutionized science world and more particularly cellular and molecular biology.

The technology CRISPR-Cas9 allows genetic editing through mutation. For example this leads to the generation of Knock-In or Knock-Out mice for animal studies [1]. Introducing mutation requires some controls to check the validity and efficiency of the mutation with classic method like PCR, sequencing [2], Western Blot, cell-based assays…

Precellys® is then used, as the gold standard to perform sample disruption to grind tissue or cells. Complete homogenization is needed to extract high quality DNA, RNA or protein for further downstream processing. The Precellys® Evolution combined to dedicated lysing kit, is the best partner to save time and improve efficiency of sample preparation protocol.

Not only for CRISPR-cas9 but in every molecular and cellular studies, quality of DNA, RNA or any other molecule of interest is critical. The Precellys® can efficiently homogenize any sample type while maintaining reproducibility amongst biological replicates and eliminating cross-contamination. This instrument is frequently used on research protocols ensuring a fast and efficient sample disruption process.

Find the related articles with a combination of CRISPR-Cas9 and Precellys® homogenizers:
[1] Genome editing with CRISPR/Cas9 in postnatal mice corrects PRKAG2 cardiac syndrome
[2] Targeted mutagenesis in Atlantic salmon (Salmo salar L.) using the CRISPR/Cas9 system induces complete knockout individuals in the F0 generation